Anti-F-Actin抗体纤维状肌动蛋白F-Actin主要存在于横纹肌原肌纤维细丝及平滑肌中，是肌肉运动和细胞运动的重要蛋白。

产品编号 yb-1571R
英文名称 Anti-F-Actin抗体
中文名称 纤维状肌动蛋白抗体
别    名 f actin; Filamentous actin; F-actin capping protein alpha subunit; CapZ alpha-1; CAZA1_HUMAN; CAPZA1.
Anti-F-Actin抗体    
说 明 书 0.1ml  0.2ml  
研究领域 免疫学  细胞骨架  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Sheep, 
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 33kDa
细胞定位 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human F-actin capping protein alpha subunit
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
产品介绍 background:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells .G-Actin (globular actin) with bound ATP can polymerise to form F-Actin (Filamentious Actin). Actin monomers spiral ariound the axis of the filament similar to a double helix. F-Actin may also undergo a process called treadmilling, in which filament length remains constant and actin monomers add at one end and dissociated at the other.

Function:
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.

Subunit:
Heterodimer of an alpha and a beta subunit. Interacts with S100A (By similarity). Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASH1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), FAM21 (FAM21A, FAM21B or FAM21C), KIAA1033, KIAA0196 and CCDC53. Interacts with S100B.

Subcellular Location:
Cytoplasm, cytoskeleton.

Similarity:
Belongs to the F-actin-capping protein alpha subunit family.


纤维状肌动蛋白F-Actin主要存在于横纹肌原肌纤维细丝及平滑肌中，是肌肉运动和细胞运动的重要蛋白。