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C反应蛋白(CRP)抗体产品应用 WB, ICC, IHC-P, IHC-F, ELISA 规格 100ug
标记物 未标记 价格 请咨询当地代理商
浓度 200ug/ml
克隆类型 多克隆 宿主 Rabbit,兔
Polyclonal Antibody to C Reactive Protein (CRP)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Rattus norvegicus (Rat)
C反应蛋白(CRP)抗体
Clonality Polyclonal
Host Rabbit
Immunoglobulin Type IgG
Purification Affinity Chromatography
Applications WB, ICC, IHC-P, IHC-F, ELISA
Concentration 200ug/ml
UOM 100ug
Conjugate No Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant CRP (His20~Ser230) expressed in E.coli.
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CRP. It has been selected for its ability to recognize CRP in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
C反应蛋白(CRP)抗体Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4,
containing 0.02% NaN3, 50% glycerol.
IHC Images
QUALITY CONTROL
Content: The quality control contains recombinant CRP (His20~Ser230) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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